Immunohistochemical stain for immunoglobulins and complements

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From:muhaizan <>
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Can anybody give his/her opinion on how to optimise immunohistochemical
staining for immunoglobulins and complements on renal tissue. My effort is
still doesn't come to success eventhough I've tried various dilution of
primary antibody and various timing for the antigen retrieval method. Non
specific staining especially for the endothelial cells is still there. Even
when I compare with the immunofluorescence findings the negative
immunofluorescence give positive results on immunohistochemistry. I used
Envision system that suppose to get rid of non specific staining in the
background caused by endogenous Biotin as compare with LSAB kit. The primary
antibody we use is from Dako.

Thank you.

Wan Muhaizan Wan Mustaffa
Dept of Pathology
Hospital University Kebangsaan Malaysia
Jalan Yacob Latiff, Bandar Tun Razak, Cheras,
56000, Kuala Lumpur, Malaysia.

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