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From:"Philopena, Jennifer" <jennifer.philopena@canji.com>
To:"'Histonet'" <histonet@pathology.swmed.edu>
Content-Type:text/plain; charset="windows-1252"

Hi.  I use citrate buffer from Zymed, in the microwave, for antigen
retrieval.  We look at p53, E1A, IL-10, IFNa transgene expression.  I have
never checked the temperature of the buffer during the process, and just
boil the slides in the solution for 30 seconds, then turn it off for 2
minutes, then on again for 30s.  I cycle like this about 10 times.  I
thought citrate buffer boiled at 95C anyway, so to check the temp is moot.

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