GFP antibodies?
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From: | Tracy Bartolotta <tbartolo@med.unc.edu> |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
I have an investigator who would like to try the Living Colors Peptide
Antibody (cat.# 8367-1 from Clontech). He wants to use it to detect
Green Fluorescent Protein using DAB instead of it's fluorescence. We
have found a paper that got this antibody to work on 4% PFA fixed frozen
tissue. Does anyone know if it will also work on paraffin embedded
tissue? Or, if you have experience with another antibody to detect GFP
I'd be interested. I know this has been discussed before on histonet,
but I can't seem to access the archives with the url address that I
have. Thank you.
Tracy Bartolotta
UNC Cystic Fibrosis Center
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