Fwd: Re: Acetylcholinesterase

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From:Paul Klosen <klosen@neurochem.u-strasbg.fr>
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>Date: Thu, 04 May 2000 13:09:17 +0200
>To: Buttigieg Carmen at MOH <carmen.a.buttigieg@magnet.mt>
>From: Paul Klosen <klosen@neurochem.u-strasbg.fr>
>Subject: Re: Acetylcholinesterase
>-At 09:57 04/05/00 +0200, vous avez écrit:
>>Dear Histonetters
>>Please help!
>>One of our pathologists just asked for a new procedure which I have never=20
>>before. He wants to stain up nerve fibres and cells containing
>>acetylcholinesterase on frozen sections of intestine.
>>I found a method in an old book where the incubation takes between 7 and 30
>>days! Is there anything simpler?
>It's been a few years since I've done AChE histochemistry, but there are 
>certainly many protocols a lot shorter than the one you mentionned. 
>Personnally, I prefered the protocol published by Di Patre et al. in JHC 
>41 (1993): 129-135. Very sensitive, easy and most reagents are cheap. I 
>have a protocol, but unfortunately it's in French. Otherwise I can fax you=20
>the material and methods section of the reprint.

                                                                        (o -) O
Paul Klosen, PhD
CNRS UMR 7518 Neurobiologie des Fonctions Rythmiques et Saisonnieres
Universite Louis Pasteur  12, rue de l'Universite
F-67000 Strasbourg, FRANCE
Tel.  Fax.

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