Fw: question on histology
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From: | LEROY BROWN <lhbhcs@pioneernet.net> |
To: | histonet <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1" |
A friend has asked the following question, anyone know what to advise on
the question?
I was wondering if you might help me answer a relatively simple question
on histology technique. I've got a protocol that is for staining and
dehydrating nissl sections for brain tissue. It goes through several
steps, starting by dehydrating and then soaking in 50/50 CHCl4/100%EtOH,
then hydrating to stain, then clearing, and dehydrating and finally
soaking in xylene before coverslipping.
What are the purposes of using chloroform and xylene in clearing/staining
tissues? Do they serve different purposes? Do you know what they actually
do to the tissue, chemically? Any help you could give me would be wonderful
and informative.
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