Endogenous peroxidase - Major incompatibilities

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From:George King <pat055@abdn.ac.uk>
To:Histonet <histonet@Pathology.swmed.edu>
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Hi there

In the Histonet digest I have been following the recent discussion on
endogenous peroxidase blocking methods.  One of the suggested methods
involved mixing acetone and hydrogen peroxidase to deal with fixation
and blocking in a single step.  The mixing of acetone and any strong
oxidising agent (including hydrogen peroxide) is to be avoided, this is
listed in an old BDH handbook which I have as one of the major
incompatibilities likely to cause violent reactions.

George King
University of Aberdeen
Department of Pathology
Aberdeen, AB25 2ZD
Tel. (0)1224 681818 x52842
Fax. (0)1224 663002

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