Hi histonetters (especially Zenobia downunder),

I've got a problem with our MSA (muscle specfic antigen). It is producing a
lot of background. The antibody is has been  frozen in aliquots , so I don't
think that the problem can be contamination. I have followed the spec sheet
recommendations of using Tris EDTA buffer, but all to no avail. I have
always understood that freezing an antibody prolongs its shelf life
(indefinately according to some) but could degradation account for the
background? Any insights?

Many thanks
Louise taylor



Research Laboratory
Department of Anatomical Pathology
South African Institute for Medical Research
Johannesburg
South Africa