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From:Buttigieg Carmen at MOH <>
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Dear Histonetters

Please help!

One of our pathologists just asked for a new procedure which I have never done 
before. He wants to stain up nerve fibres and cells containing 
acetylcholinesterase on frozen sections of intestine.
I found a method in an old book where the incubation takes between 7 and 30 
days! Is there anything simpler? 
If anyone has the protcol could you please also send catalogue numbers of the 
not so common reagents which would certainly be involved.
Thanks for your help.

St. Luke's Hospital
Fax: 0356 224286

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