p21

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From:Liz Sabin <esabin@srv4.med.ed.ac.uk>
To:HistoNet@Pathology.swmed.edu
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Date:Fri, 19 Mar 1999 10:41:07 +0000
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Hello all,

Does anyone have any experience in immunohistochemical staining for 
p21 and p53 in formalin fixed human tissue?  I'd like to know if, and 
to what extent,  microwave antigen retrieval can lead to unreliable 
results. ie false positives.  Can anyone suggest an example of an 
appropriate negative control human tissue to run along side my test 
sections?  I'm already using an isotype control for the antibody.

Thanks,

Liz.


Liz Sabin
Department of Pathology
University of Edinburgh
Medical School
Teviot Place
Edinburgh
EH8 9AG

email. esabin@srv4.med.ed.ac.uk




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