long-term tissue storage

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From:"Karen D. Larison" <LARISONK@UONEURO.uoregon.edu>
Date:Tue, 16 Mar 1999 16:10:45 -0800


I believe Ian Montgomery asked about methods for long-term tissue storage that 
would preserve antigenicity.  One of the PIs here has been storing his 
embryonic rat brains at -20C in ethylene glycol-based cryoprotectant.  He finds 
that the brains he has stored this way can be processed for IHC and in situ 
hybridization.  He claims that in some cases the staining of brains stored in 
this manner is superior to that of brains that are processed immediately 
following fixation.  Two references for this method:  Peptides 7, 155-159, 
1986; Brain Research 578, 155-160, 1992.  In the Peptides paper, the authors 
were even able to get decent electron micrographs of tissue that had been 
stored in this manner.

Good luck.

Karen Larison -- University of Oregon   

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