long-term tissue storage
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From: | "Karen D. Larison" <LARISONK@UONEURO.uoregon.edu> |
To: | HistoNet@Pathology.swmed.edu |
Reply-To: | |
Date: | Tue, 16 Mar 1999 16:10:45 -0800 |
Content-Type: | |
Histonetters,
I believe Ian Montgomery asked about methods for long-term tissue storage that
would preserve antigenicity. One of the PIs here has been storing his
embryonic rat brains at -20C in ethylene glycol-based cryoprotectant. He finds
that the brains he has stored this way can be processed for IHC and in situ
hybridization. He claims that in some cases the staining of brains stored in
this manner is superior to that of brains that are processed immediately
following fixation. Two references for this method: Peptides 7, 155-159,
1986; Brain Research 578, 155-160, 1992. In the Peptides paper, the authors
were even able to get decent electron micrographs of tissue that had been
stored in this manner.
Good luck.
Karen Larison -- University of Oregon
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