From: | "Instrumedics, Inc." <info@instrumedics.com> |
To: | HistoNet Server <HistoNet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Tue, 30 Mar 1999 13:57:21 -0500 |
Content-Type: | text/plain; charset="iso-8859-1" |
Kelly asked how to maintain her brain cell pellet frozen for cryosectioning. If you have to freeze the cells in the centrifuging step Instrumedics has an embedding medium which freezes at -10deg.Celsius. It is an oil that we keep in a thermostatted bath in the cryostat so that it is at -8deg and liquid. The frozen tissue or pellet will not melt when you place it in the oil in an embedding mold. You then freeze the oil and the tissue inside the cryostat The frozen oil sections well. The cold, liquid oil is also an excellent way to avoid dehydration of the tissue during storage in a freezer. The oil is brushed on the surface of the block and the block will be protected from dehydration for many months. The oil is also used to repair a damaged block without melting the tissue. The Protective Oil Accessory Kit is an option when the CryoJane system is purchased. If you want more details please contact Instrumedics. Bernice schiller@instrumedics.com 800-237-2772