cryo sample - problem
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From: | Kelly Randall <kellyr@neuro.hfh.edu> |
To: | HistoNet@Pathology.swmed.edu |
Reply-To: | |
Date: | Mon, 29 Mar 1999 15:43:01 -0500 (EST) |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
We are trying to cryosection samples of brain cells that have been spun
down in a centrifuge tube. The frozen pellets are "popped" out of
the tube, but they begin to thaw when the embedding media is added.
If we tried to use a thinner walled centrifuge tube would it be possible
to section the pellets AND the tube together? We have a cryotome
that used disposable blade, and another that has a stainless blade. I
guess that this is quite possible right, since many of you cut bone, but
would like some tips if anyone has them to offer.
Thanks in advance!
Kelly
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