cryo sample - problem

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From:Kelly Randall <>
Date:Mon, 29 Mar 1999 15:43:01 -0500 (EST)
Content-Type:TEXT/PLAIN; charset=US-ASCII

We are trying to cryosection samples of brain cells that have been spun 
down in a centrifuge tube.   The frozen pellets are "popped" out of 
the tube, but they begin to thaw when the embedding media is added.
If we tried to use a thinner walled centrifuge tube would it be possible 
to section the pellets AND the tube together?  We have a  cryotome 
that used disposable blade, and another that has a stainless blade.  I 
guess that this is quite possible right, since many of you cut bone, but 
would like some tips if anyone has them to offer.

Thanks in advance!

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