capillary gap technology
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From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Tue, 23 Mar 1999 11:42:21 -0700 |
Content-Type: | |
Biotek is a huge automated version of the Microprobe, and now sold
by Ventana. Fisher is also touting the capillary gap system as a way
to perform routine and special stains in addition to immunohistochemistry
which is how our labs utilizes it. It is very cost effective in terms of
reagents, slides are a bit more expensive, but wonderful for doing larger
volumes of slides (20 per holder) with options to stain more than one
antibody at a time. It takes some practice to use it, but as in all things,
easy to learn and work with. It certainly is a nice inexpensive semiautomated
way to do large numbers of immunostains, and compatible with kit use.
Zymed has reagents designed for use with capillary systems, CapGap??, hope
I got that right?
It works well, and there is a wonderful article in Journal of Histotechnology
by John Frank et al on Cost and time saving immunohistochemical method
for co-localization of prolactin with growth hormone or adrenocorticotropic
hormone in rat pituitary, Dec 1997 20:313-319.
John works with this system about as well as anyone I know. I know it can
be used for apoptosis TUNEL methods (Trevingen). I thought John's setup was
exceptional for ease of handling slides, etc and his method has become the
gold standard for our setup.
Gayle Callis
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