I have sections that are of cross sections of rat heads
and a recent batch came back with the tissues all wavy
on the slide.  They were fixed in Zinc Formalin (4d)
then decalcified in 13% Formic Acid for 4d.  The bone
is all intact in the section (no shattering, cutting
was easy) so I think the tissues were adequately
decalcified.  However, our protocol calls for a 4 to 7
day decal, and these rat heads were a bit on the larger
side, so I'm wondering if the tissues were just
slightly under decalcified.  Any suggestions I can pass
onto our histo lab to help them achieve flat
sections??

===
Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

*new* e-mail: bresee98@yahoo.com

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