Speedy Decal
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From: | "Johnson, Jennifer(Hist)" <jjohnson3@genzyme.com> |
To: | "'histonet'" <histonet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Fri, 26 Mar 1999 13:28:19 -0500 |
Content-Type: | |
Hi Histonetters,
I am interested in finding out any information on speeding up the
decal process (including microwaving). Right now my lab is using Shandon's
TBD-2, which is a 26% Formic Acid in a Sodium Citrate Buffer. The decal is
used on a variety of sizes up to 1"x1". The tissue is fixed in 10%NBF for 7
days and submerged in decal. Every 48 hours we check the progress by mixing
5ml's of spent decal with 1ml of 5% Ammonium Oxalate. If a precipitate
forms the decal is changed and checked again until no precipitate forms. We
have chosen this method for it's ability to maintain antigenicity of the
tissue for collage type I & II. It does however take a good deal of time.
If anyone has any other ideas of how to speed this up (but maintain quality
and antigenicity) I would appreciate it. Thanks!!
* Jenn Johnson
* Principal Research Associate
* Genzyme Corporation
* P.O. Box 9322
* Framingham MA 01701
* ph-508-271-3610
* jjohnson3@genzyme.com
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