Speedy Decal

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From:"Johnson, Jennifer(Hist)" <jjohnson3@genzyme.com>
To:"'histonet'" <histonet@Pathology.swmed.edu>
Date:Fri, 26 Mar 1999 13:28:19 -0500

Hi Histonetters,
	I am interested in finding out any information on speeding up the
decal process (including microwaving).  Right now my lab is using Shandon's
TBD-2, which is a 26% Formic Acid in a Sodium Citrate Buffer.  The decal is
used on a variety of sizes up to 1"x1".  The tissue is fixed in 10%NBF for 7
days and submerged in decal.  Every 48 hours we check the progress by mixing
5ml's of spent decal with 1ml of 5% Ammonium Oxalate.  If a precipitate
forms the decal is changed and checked again until no precipitate forms.  We
have chosen this method for it's ability to maintain antigenicity of the
tissue for collage type I & II.  It does however take a good deal of time.
If anyone has any other ideas of how to speed this up (but maintain quality
and antigenicity) I would appreciate it.  Thanks!!

*	Jenn Johnson
*	Principal Research Associate
*	Genzyme Corporation
*	P.O. Box 9322
*	Framingham MA 01701
*	ph-508-271-3610
*	jjohnson3@genzyme.com

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