Jim, 
You might try developing the sections on glass rods and flooding the slide
with the freshly prepared developer. Since the gelatin solidifies on my
slides during development, I would imagine that you end up with a Jello
pudding pop in your staining jar. Maybe decreased access to light rays in
the slides toward the center of your staining vessel is causing the uneven
staining results. 

Jeff Silverman 
Southampton Hospital NY USA
peptolab@hamptons.com

----------
> From: Jim Ball <xryhisto@ovis.net>
> To: histonet@Pathology.swmed.edu
> Subject: warthin-starry
> Date: Thursday, March 18, 1999 4:16 PM
> 
> was wondering if I might be able to pick up some pointers on what I refer
to
> as worthless-starry procedure. I have no problem staining the organism,
but
> find the over all staining of the tissue as being my back ground stain(
the
> actual gold to brown counter stain imparted by the sliver) to be less
than
> desirable. I ran 5 positive control slides today and developed them for
the
> same length of time in a glass coplin jar.
> the color contrast ran the gamit from 1 slide staining perfectly to 2
slides
> being marginaly acceptable to 2 slides being total worthless(hinse my pet
> name for the stain). I have found the imprenation step  to be the most
> improtant to maintaining the organisms distintive shape.
>          I guess the thing that concens me the most is that the mucus
> producing areas of the biopsy seems to get overly dark and may cause
false
> negative results since in cases where there are only a few organisms
present
> these dark staining areas could mask the organism, and cause a false
> negative report to be generated.
>          Please foward all formulas for cleaning glass ware as this may
be
> the problem and many of my fellow techs seem to think that rinsing the
glass
> ware in several changes of DI will cure all. I am of the opinion that
> disposable plastic is the way to go. My best results were obtained using
the
> plastic disposable urine containers and Plastic slide mailers. The slide
> mailers were used only one time and then recyled as what other than slide
> mailers after being thourghly disenfected.
>           After writing this I may have come up with something else to
try,
> god it helps to pour out your heart to a caring audiance, if only you
could
> despense a drink over the net what great bartenders we would make. Hope I
> have made enough speeling errors for all the speelin bee weenies out
there
> In the words of that great actor" frankly Scarlet I don,t give a damn."
The
> day has been way to long and I need that dink I was talking about
earlier.
>