Dear Kathleen,
    Your message implies on some days (all) biopsies are over dehydrated and
some days (all) biopsies process fine.   Is this the case?  If processing
yields
the same results on all specimens on a particular day, make note of that day.  
Do this for a couple of weeks.  If you find that the day following reagent
changes yield more of these negative symptoms and the day the reagents are most
diluted yield desired processing results - it would appear that the dehydration
steps are either too long or too high of a concentration.   
    
    If such a pattern is not demonstrated, and only a portion of the specimens
are afflicted by these negative symptoms per run, investigate variables prior
to
your receipt of the specimens.

Kind regards,

Nancy

Nancy Klemme, HT(ASCP)
Customer/Product Support Mgr.
Sakura Finetek USA, Inc.
1750  West 214th Street
Torrance, CA  90501

Web Page = www.sakuraus.com 
e-mail = nancy.klemme@sakuraus.com
Phone = 800/725-8723

____________________Reply Separator____________________
Subject:    processing GI biopsies
Author: "Kathleen Hollenbeck" <lastandfirst@webtv.net>
Date:       3/25/99 8:41 PM

From: Kathleen Hollenbeck
Date: Thu, Mar 25, 1999 8:41 PM
Subject: processing GI biopsies
To: histonet
We process our GI biopsies on a short cycle using a VIP tissue
processor.  Our results are not consistent.  Somedays we experience a
lot of microchatter and other days we do not.  We receive specimens from
out-patient locations in addition to our in-house surgical specimens.

Any helpful hints will be greatly appreciated.  Thanks in advance.

Kathleen Hollenbeck
Histology Supervisor
Sunrise Hospital
Las Vegas, NV



----------
Received: from email.swmed.edu by powell.fabrik.com
        with SMTP (Fabrik F07.3-000)
        id SINN.12739318@powell.fabrik.com ; Thu, 25 Mar 1999 20:52:05 -0800
Received: from 129.112.18.39 ([129.112.18.39])
 by SWAX32.SWMED.EDU (PMDF V5.1-8 #18228)
 with SMTP id <01J99IM2K8LUA9NNYR@SWAX32.SWMED.EDU> for
 nancy.klemme@sakuraus.com; Thu, 25 Mar 1999 22:51:44 CST
Date: Thu, 25 Mar 1999 20:41:54 -0800 (PST)
From: lastandfirst@webtv.net (Kathleen Hollenbeck)
Subject: processing GI biopsies
To: histonet@pathology.swmed.edu
Message-id: <773-36FB1012-3478@mailtod-172.iap.bryant.webtv.net>
MIME-version: 1.0 (WebTV)
Content-type: Text/Plain; Charset=US-ASCII
Content-transfer-encoding: 7Bit

----------