Kathleen,

Here a few suggestions.

Check the temperature of the paraffin baths.  Try using a low melting point
paraffin.  Also, make sure all fluids are at ambient temp.

To help out with chatter, rough cut blocks and place on ice that has begun
to melt.  This will soften the tissue.

Rande Kline HT (ASCP)
Technical Services
EM Science




lastandfirst@webtv.net (Kathleen Hollenbeck) on 03/25/99 11:41:54 PM

To:   histonet@pathology.swmed.edu
cc:
Subject:  processing GI biopsies




We process our GI biopsies on a short cycle using a VIP tissue
processor.  Our results are not consistent.  Somedays we experience a
lot of microchatter and other days we do not.  We receive specimens from
out-patient locations in addition to our in-house surgical specimens.

Any helpful hints will be greatly appreciated.  Thanks in advance.

Kathleen Hollenbeck
Histology Supervisor
Sunrise Hospital
Las Vegas, NV