>
>You get massive to moderate hemorrhage in the lungs and organs with CO2 so
>would not be surprised if you did get alot of formyl hemoglobin on
>fixation.  Euthanasia with a euthanasia drug like Beuthanasia will give you
>crystalline deposits in tissue.  I recommend pentobarbital IP or IV for
>least amount of artefact.
>
>Dr. Annette Gendron
>>>
>>>A question for the group.
>>>I'm planning an experiment in which mice will be killed and the
>>>lungs and several other organs examined histologically.  Some of
>>>the mice will be transgenic.  The question is, would killing the
>>>animals with CO2 narcosis have an adverse impact on the quality
>>>of preservation, e.g. generating formalin pigment in the spleen?
>>>Does anyone have experience with this method?
>>>
>>>Thanks in advance
>>>
>>>Hal Hawkins
>>>UT Medical Branch, Galveston, TX
>>>
>>
>>
>>
>>	~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~	Ma

>>>
>>>ureen Decorah, Research Animal Resources Center
>>	385A Enzyme Institute, 1710 University Avenue
>>	University of Wisconsin - Madison, Madison, WI 53705-4098
>>	(608) 262-0933  Fax(608) 265-2698  E-mail: decorah@rarc.wisc.edu
>>
>>
>>	~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~u�
>
>
>
>
>*******************************************************************************
>Dr.Annette Gendron-Fitzpatrick                          Rm. 380 Enzyme
>Institute
>Research Animal Resource Center                         1710 University Ave.
>(608) 262-1239, gendron@rarc.wisc.edu                   Madison, WI 53705-4098
>*******************************************************************************
>
>

	~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
	Ma
ureen Decorah, Research Animal Resources Center
	385A Enzyme Institute, 1710 University Avenue
	University of Wisconsin - Madison, Madison, WI 53705-4098
	(608) 262-0933  Fax(608) 265-2698  E-mail: decorah@rarc.wisc.edu
	~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

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