Re: preservation of mice
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From: | Maureen Decorah <decorah@rarc.wisc.edu> |
To: | HISTONET <HISTONET@Pathology.swmed.edu> |
Reply-To: | |
Date: | Wed, 31 Mar 1999 07:03:26 -0600 |
Content-Type: | text/plain; charset="iso-8859-1" |
>
>You get massive to moderate hemorrhage in the lungs and organs with CO2 so
>would not be surprised if you did get alot of formyl hemoglobin on
>fixation. Euthanasia with a euthanasia drug like Beuthanasia will give you
>crystalline deposits in tissue. I recommend pentobarbital IP or IV for
>least amount of artefact.
>
>Dr. Annette Gendron
>>>
>>>A question for the group.
>>>I'm planning an experiment in which mice will be killed and the
>>>lungs and several other organs examined histologically. Some of
>>>the mice will be transgenic. The question is, would killing the
>>>animals with CO2 narcosis have an adverse impact on the quality
>>>of preservation, e.g. generating formalin pigment in the spleen?
>>>Does anyone have experience with this method?
>>>
>>>Thanks in advance
>>>
>>>Hal Hawkins
>>>UT Medical Branch, Galveston, TX
>>>
>>
>>
>>
>> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Ma
>>>
>>>ureen Decorah, Research Animal Resources Center
>> 385A Enzyme Institute, 1710 University Avenue
>> University of Wisconsin - Madison, Madison, WI 53705-4098
>> (608) 262-0933 Fax(608) 265-2698 E-mail: decorah@rarc.wisc.edu
>>
>>
>> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~u
>
>
>
>
>*******************************************************************************
>Dr.Annette Gendron-Fitzpatrick Rm. 380 Enzyme
>Institute
>Research Animal Resource Center 1710 University Ave.
>(608) 262-1239, gendron@rarc.wisc.edu Madison, WI 53705-4098
>*******************************************************************************
>
>
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Ma
ureen Decorah, Research Animal Resources Center
385A Enzyme Institute, 1710 University Avenue
University of Wisconsin - Madison, Madison, WI 53705-4098
(608) 262-0933 Fax(608) 265-2698 E-mail: decorah@rarc.wisc.edu
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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