Re: cryo sample - problem
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From: | Jamie Erickson <JErickson@genetics.com> |
To: | kellyr@neuro.hfh.edu, HistoNet@Pathology.swmed.edu |
Reply-To: | |
Date: | Wed, 31 Mar 1999 10:10:56 -0500 |
Content-Type: | |
Kelly I did what you are doing with CHO cells pelleted. I pelleted the cells and then put the 15 ml conicle tip under liquid nitrogen for about 1 minute, inverted the tube and popped out the pellet. I then froze the pellet in OCT. The pellet did melt a little but I was able to cut the pellet and do immuno on it. I used a lot of cell 10 million/ml the pellet was large. Hope it helps.
Jamie Erickson
Genetics Institute
Andover, MA
>>> Kelly Randall <kellyr@neuro.hfh.edu> 03/29 3:43 PM >>>
We are trying to cryosection samples of brain cells that have been spun
down in a centrifuge tube. The frozen pellets are "popped" out of
the tube, but they begin to thaw when the embedding media is added.
If we tried to use a thinner walled centrifuge tube would it be possible
to section the pellets AND the tube together? We have a cryotome
that used disposable blade, and another that has a stainless blade. I
guess that this is quite possible right, since many of you cut bone, but
would like some tips if anyone has them to offer.
Thanks in advance!
Kelly
!
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