Kelly,

Are you using OCT for embedding? Try this: Spin down to a pellet. 
Re-suspend in OCT. Spin down again and freeze. Now when you remove the  
pellet from the tube the pellet is ready to attach to a cryo-chuck.

Tim Morken, B.A., EMT(MSA), HTL(ASCP) 
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

FAX:  (404)639-3043



----Original Message Follows----
From: Kelly Randall <kellyr@neuro.hfh.edu>
To: HistoNet@Pathology.swmed.edu
Subject: cryo sample - problem
Date: Mon, 29 Mar 1999 15:43:01 -0500 (EST)


We are trying to cryosection samples of brain cells that have been spun 
down in a centrifuge tube.   The frozen pellets are "popped" out of 
the tube, but they begin to thaw when the embedding media is added.
If we tried to use a thinner walled centrifuge tube would it be possible 
to section the pellets AND the tube together?  We have a  cryotome 
that used disposable blade, and another that has a stainless blade.  I 
guess that this is quite possible right, since many of you cut bone, but 
would like some tips if anyone has them to offer.

Thanks in advance!
Kelly  





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