I'd like to add my two cents.  Use these products with gloves.  Propidium 
iodide and DAPI are nasty, particularly the DAPI, which readily passes through 
cellular membranes. I also think that the antifade reagent in Vectashield may 
be nasty.  I always caution people to use gloves when using this product.

Karen Larison - University of Oregon


Date:          Wed, 31 Mar 1999 11:11:37 -0800
From:          Ronnie Houston <rhh1@airmail.net>
Subject:       Re: Vectsashield w/ DAPI?
To:            "John C. Dennis" <dennijc@vetmed.auburn.edu>
Cc:            histonet@pathology.swmed.edu

John,
Yes, both the DAPI and Propidium iodide work really well. They preserve
the immunofluorescence over an extended period (have re-examined
sections one month after initial mounting - sections stored in dark at
4C).
One cautionary note, from my experience, is that it is important to
photograph your results within a day or so, as the DAPI and Propidium
iodide tend to bleed slightly, making nuclei appear "fuzzy".
Ronnie


John C. Dennis wrote:
> 
> Ronnie Houston
> 
> Have you tried this mounting medium.  Does it work?
> 
> John Carroll Dennis
> Anatomy, Physiology, and Pharmacology
> 109 Greene Hall
> Auburn University, AL  36849
> 
> On Wed, 31 Mar 1999, Ronnie Houston wrote:
> 
> > Steven,
> > I think what you are referring to is Vectashield mounting medium with
> > DAPI from Vector Laboratories, cat# H-1200. It fluoresces nuclei blue.
> > They also make Vectashield with Propidium Iodide which fluoresces red.
> > Ronnie Houston
> > Cytochemistry & Molecular Pathology
> > Texas Scottish Rite Hospital for Children
> > 2222 Welborn Street
> > Dallas
> >