Dear Lori and Histonetters:
I have reference information regarding a "neat" technique of lifting tissue
sections or stained smears from slides and replacing the specimens on new
slides.  Multiple slides of a highly cellular smear can be prepared for study.
I have two references that outline or demonstrate the technique and there
is another one by Parula Mehta published in Applied Immunohistochemistry,
but the exact reference I don't have at this time.

References:
Jimenez-Joseph D, Gangi MD: Application of diatex compound in cytology: Use
in preparing mulitple slides from a single routine smear. Acta Cytol
30:445, 1986.

Sherman ME, Jimenez-Joseph D, Gangi MD, Rojas-Corona, RR: Immunostaining of
Small Cytologic Specimens-Facilitation with Cell Transfer. Acta Cytol
38:18-22, 1994.

I can fax copies if you send an e-mail request.

Histologically yours,
Daisy


At 03:36 PM 3/14/99 -0800, you wrote:
>hello, my name is not Lori, but I would be interested in you faxin a 
>copy ot "How to do Multiple Immunstains When Only One Tissue Slide is 
>Available" my fax # is 303 -316-9084.  Thank you in advance,
>Sonja DiGiacomo, Prostate Diagnostic Lab, Denver, CO.
>
>
>>From: rkline@emindustries.com
>>To: lmiller@wlgore.com
>>CC: Histonet@pathology.swmed.edu
>>Subject: Re: Saving broken slide tissue
>>Date: Fri, 12 Mar 1999 15:10:46 -0500
>>
>>Lori,
>>
>>If you would like a copy of " How to Do Multiple Immunostains When Only 
>One
>>Tissue Slide Is Available " send me your fax number.  The product 
>mentioned
>>in the article is manufactured by EM Science and can be purchased 
>through
>>most of the distributors.
>>
>>Krystalon
>>64969/71  16oz
>>64969/95  6x2ml  (these are small bottles with applicators)
>>
>>Rande Kline HT( ASCP)
>>Technical Services
>>EM Science
>>
>>
>>
>>
>>
>>lmiller@wlgore.com on 03/12/99 11:30:12 AM
>>
>>To:   Histonet@pathology.swmed.edu
>>cc:
>>Subject:  Saving broken slide tissue
>>
>>
>>
>>
>>
>>
>>Can anyone tell me how to remove tissue from a broken slide and mount 
>it
>>onto another slide? I've heard of this being done, but never actually 
>seen
>>the technique.
>>Thanks!
>>Lori
>>
>>
>>
>>
>>
>>
>>
>>
>
>Get Your Private, Free Email at http://www.hotmail.com
>
>