Re: Elastic stains and Myelin
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From: | FreidaC@aol.com |
To: | lbyano@bpthosp.org, histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Thu, 18 Mar 1999 14:12:44 -0500 (EST) |
Content-Type: | text/plain; charset=US-ASCII |
Bonnie:
Puchtler and Waldrop published "On the Mechanism of Verhoeff's Elastica Stain:
A Convenient Stain for Myelin Sheath" in Histochemistry 62:233-247 (1979).
They state "Verhoeff's elastica stain is definitely not specific for elastin
and is inferior to orcein and resorcin-fuchsin because of the required
differentiation with its inherent bias to produce patterns which conform to
expectations. However, Verhoeff's elastica stain is far superior to other
metal-hematein technics for myelin sheaths. The combined Verhoeff-picro-Sirus
Red F3BA stain can be performed in 30 min and does not require
differentiation. It is therefore suggested to reclassify Verhoeff's elastica
stain as a method for myelin sheaths."
Wonder why this did not seem to go anywhere? Hope this helps, and that your
pathologists can find the reference. I have a copy of the paper if all else
fails.
Freida Carson
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