Melissa Brennan wrote:
> 
> Does anyone out there know anything about doing IHC on skin? Any published
> articles?
> 
> Melissa Brennan
> Silver Spring, MD
> 
> -----Original Message-----
> From: HistoNet Server [mailto:HistoNet@Pathology.swmed.edu]
> Sent: Saturday, March 27, 1999 11:00 PM
> To: HistoNet Server
> Subject: Daily Digest
> 
> ----------------------------------------------------------------------
> 
> Date: 27 Mar 1999 00:11:07 -0600
> From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
> Subject: Silver stain removal (& other thoughts. Was: Thanks)
> 
> On Fri, 26 Mar 1999, Inka Tertinegg wrote:
> 
> > Does anyone know how to get silver stain out of
> > a white benchtop?
> 
>   Inka:  It depends how tough the bench top is.
> 
>     1. Nitric acid will remove silver (but not gold).
>        Dilute the conc. acid about 50/50 with water.
>        OK on epoxy paint. I don't know about formica,
>        but would guess probably safe at this dilution.
> 
>     2. (From back in the old days) A paste of mercuric
>        chloride and water rubbed into the black silver
>        stain. More gentle on the skin than nitric acid,
>        I've been told. Never really an acceptable method.
> 
>     3. Farmer's reducer, as used in photography; but for
>        cleaning purposes it needs to be more concentrated.
>        Crunch up about one cubic centimetre each of
>        potassium ferricyanide and sodium thiosulphate in
>        15-20 ml of water. Apply generously to the blackened
>        bits of bench. It will remove the silver but not
>        quickly. Allow 30-60 minutes and don't let it dry
>        out. The slowness is a penalty for (a) spilling
>        your expensive silver solution, and (b) using fairly
>        harmless chemicals.
>          Be sure to use ferrIcyanide and NOT ferrOcyanide,
>        which will not work. Despite the -cyanide in their
>        names, these are not considered dangerous poisons.
> 
>          (Cyanides, well known for their speed, are much more
>           potent and also easily available to murderers in
>           detective stories. When I was a lad, one of the
>           other students in the lab needed to order some
>           KCN for histochemical purposes. He needed about
>           50 mg but the stuff was cheap and the smallest
>           bottle held 500 g. We looked it up in Polson's
>           Toxicology and found that this would be enough to
>           kill 1500 people, if they could all be
>           persuaded to swallow their 333 mg helpings. The
>           smell must surely make cyanide a rather poor
>           weapon for the enterprising poisoner: "bitter
>           almonds" to the novelist, but "sickly sweet" to
>           anyone working with amounts well below 10% of what's
>           supposed to be lethal.)
> 
>     4. None of these methods will remove dark stains due to
>        metallic gold, from spilt "gold chloride."  Cyanide
>        and oxygen would, and so would aqua regia, but these
>        are not for sploshing on the bench's top. To hide
>        carelessness with gold, you'll need a pot of paint.
> 
>            John A. Kiernan,
>            Department of Anatomy & Cell Biology,
>            The University of Western Ontario,
>            LONDON,  Canada  N6A 5C1
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 27 Mar 1999 05:30:29 -0600
> From: marsdijk@wxs.nl
> Subject: usersr of PAPnet
> 
> Are there any users of the PAPnet, and what are they going to do now NSI
> doesn't do to well ?
> 
> - --
> met vriendelijke groet,
> 
>  John
> 
> *-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*
> 
> |  John van Marsdijk            |
> 
> *  email: marsdijk@wxs.nl       *
> 
> |  Veenslag 31                  |
> 
> *  3905 SJ  Veenendaal          *
> 
> |  The Netherlands  |
> 
> *                               *
> 
> |  tel:   (+31) 06-53 44 2176   |
> 
> *-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*
> 
> ----------------------------------------------------------------------
> 
> Date: 27 Mar 1999 07:45:39 -0600
> From: Valleygal@aol.com
> Subject: ASH Workshop
> 
> The Arizona Society for Histotechnology is proud to announce the second in a
> series of workshops to be given on April 17, 1999 at Scottsdale Healthcare -
> Osborn Campus. The topic of this workshop is Her2neu by IHC, Prognostic and
> Therapeutic Values (3.0 contact hours) and is being sponsored by IMPATH and
> Dako Corp. The speaker is Daisey Jimenez Joseph, B.A., HTL (ASCP).
> The workshop will be held in the conference room at 7300 E. 4th Street and
> will begin at 10AM.
> A lunch will be served courtesy of IMPATH and Dako.
> There is no charge to ASH members and a $5.00 charge for non-members.
> RSVP by April 13, 1999 by contacting Carole Reeder at 602-481-4000, ext.
> 2199.
> Space is limited for this wet workshop and lecture so make your reservations
> early.
> See you there!
> 
> Andi Grantham
> 
> ----------------------------------------------------------------------
> 
> Date: 27 Mar 1999 08:30:59 -0600
> From: "Sandy Julsing" <sandy.julsing@unitedhealth.org>
> Subject: processing GI biopsies -Reply
> 
> Kathleen,
>    How do you warm your forceps for embedding?  Even if GI biopsies are
> fixed
> on time, processed gently and oriented carefully, if a flame or incinerator
> (400 degrees!) are used to embedd and you go directly from these over heated
> sources to your tiny unsuspecting gastric biopsy, you can sizzle you tissue
> instantly.
>     Just a thought!
> 
> >>> Kathleen Hollenbeck <lastandfirst@webtv.net> 03/25 10:41 pm >>>
> We process our GI biopsies on a short cycle using a VIP tissue
> processor.  Our results are not consistent.  Somedays we experience a
> lot of microchatter and other days we do not.  We receive specimens from
> out-patient locations in addition to our in-house surgical specimens.
> 
> Any helpful hints will be greatly appreciated.  Thanks in advance.
> 
> Kathleen Hollenbeck
> Histology Supervisor
> Sunrise Hospital
> Las Vegas, NV
> 
> ----------------------------------------------------------------------
> 
> Date: 27 Mar 1999 09:15:48 -0600
> From: "Gary W. Gill" <garywgill@email.msn.com>
> Subject: RE: usersr of PAPnet
> 
> FYI, Neuromedical Systems voluntarily filed on 03/26 for bankruptcy
> protection in a Wilmington, Delaware court and "therewith let go the
> majority of its US workforce."  NeoPath, maker of AutoCyte, will acquire
> NSI's "patent estate" and 1.4 million shares of common stock for $4 million,
> pending approval.  The recently published "Duke evidence report" and JAMA
> article (too little bang for the buck) probably accelerated the inevitable
> demise.
> 
> Perhaps some large lab will acquire numerous deeply discounted PapNet
> devices and offer a regional Pap smear screening service.  However, the
> latter is what got NSI into trouble in the first place.  Or perhaps, a few
> university researchers will use PapNet to conduct cytological studies.  But
> where will they get the spare parts?  It's not a happy situation.
> 
> I am not a PapNet user, though I took their week long training course in
> 1997.  The technology is impressive, but NSI's early marketing strategy and
> tactics were not well received by potential end users.  NSI persisted in
> this strategy for too long to recover.  Like all automated systems, PapNet
> is not perfect; it still misses abnormal cells.  It is also very expensive.
> PapNet driven screening microscopy systems are very sophisticated
> electronically and require daily QC/QA measures -- which is to be expected.
> 
> I wish all NSI employees well.  The few I met were competent, conscientious,
> personable individuals.  Having experienced sudden and unexpected
> outplacement twice in the past 10 years, I appreciate firsthand the turmoil
> created in one's personal and professional life.  Becoming re-employed is
> especially difficult when one's job skills are very specialized and matching
> job opportunities are few and far between.
> 
> Gary Gill
> 
> > -----Original Message-----
> > From: John van Marsdijk [mailto:marsdijk@wxs.nl]
> > Sent: March 27, 1999 3:11 AM
> > To: Histonet listserver
> > Subject: usersr of PAPnet
> >
> >
> > Are there any users of the PAPnet, and what are they going to do now NSI
> > doesn't do to well ?
> >
> > --
> > met vriendelijke groet,
> >
> >  John
> 
> ----------------------------------------------------------------------
> 
> Date: 27 Mar 1999 10:00:22 -0600
> From: marianne brown <mbrown_biocaremedical@yahoo.com>
> Subject: Re: CD-19
> 
> - --- "Nocito, Joseph" <joseph_nocito@srhc.iwhs.org> wrote:
> > Hello Histo land,
> > does any one know of a CD-19 antibody that works in
> > paraffin.  My
> > pathologists want another B-cell marker other than
> > CD-20.  As always, thanks
> > for your help.
> >
> > Joe Nocito, B.S., HT(ASCP)QIHC
> > Histology Supervisor
> > Christus Santa Rosa Hospitals
> > San Antonio, Texas
> Biocare Medical 1-800-799-9499 has a coctail B cell marker L26/CD20
> Marianne
> >
> >
> >
> 
> ===
> Marianne Brown
> Consultant Biocare Medical
> 
> _________________________________________________________
> Do You Yahoo!?
> Get your free @yahoo.com address at http://mail.yahoo.com
> 
> ----------------------------------------------------------------------
> 
> Date: 27 Mar 1999 10:00:51 -0600
> From: "D. Hammer" <hammerd@u.washington.edu>
> Subject: Histonet Archives
> 
> Marvin,
> 
> I just spent a great time on the site you inform us about below.
> Fantastic job!  It was so easy to use and will be so helpful to us when
> gathering info on specific topics.  I have noticed several messages on
> histonet begin with ...."I know this topic has been discussed before, but
> ......."   Now we can just go to your site and extract the info you have
> stored.
> 
> Thanks again for a terrific addition the the Histonet Service.  What a
> world we live in :)
> 
> Don
> PS  A tip for Histonetters who access the page by copying the
> address....becareful not to include the period after the address, took me
> a couple of try's before figuring out why it wouldn't open.
> 
> 
> - ---------- Forwarded message ----------
> Date: Mon, 08 Mar 1999 19:55:55 -0500
> From: Marvin Hanna <mhanna@magicnet.net>
> To: histonet@Pathology.swmed.edu
> Subject: Histonet Archives
> 
> Hi Histonetters,
> 
> We have been running a search engine for pathologists and laboratory
> professionals for the past 6 months at http://www.pathsearch.com. A few of
> my friends and co-subscribers on Histonet  have asked if we could archive
> Histonet, so...
> 
> There is now an archive of Histonet messages since October, 1998 on the web
> server at http://www.pathsearch.com/histonet.html. They are available as
> either an archive by dates or searchable by the search engine. The web
> server updates the archive hourly.
> 
> We have also searched and indexed a number of other histology resources on
> the Internet and provided a specialized search engine for them and
> Histonet. You can search sites either collectively or individually at
> http://www.pathsearch.com/histosearch.html. Histosearch is a subset of over
> 10, 000 pages that is included in the over 50,000 pages searched and
> indexed on Pathsearch.
> 
> We have also set up a 24 hour a day chat server, the ability to publish
> home pages or technical papers on the Internet in a couple of minutes, and
> intelligent agent technology for both Pathsearch and Histosearch.
> 
> The Histonet archives, Histosearch, and Pathsearch are free to
> histologists, pathologists, and laboratory professionals on the Internet.
> We do plan on offering sponsorships on Histosearch and Pathsearch to help
> cover the cost of colocating the server on a T-3 line, hardware, software
> and time.
> 
> Linda and Herb have done a great service for the field of histology in
> setting up and running Histonet. I hope archiving this information will
> benefit the participants of Histonet.
> 
> Marvin Hanna
> webmaster@pathsearch.com
> 
> ----------------------------------------------------------------------
> 
> Date: 27 Mar 1999 10:01:15 -0600
> From: "Nocito, Joseph" <joseph_nocito@srhc.iwhs.org>
> Subject: CD-19
> 
> Thanks to those who responded to my CD-19 question.  I told my pathologists
> that there wasn't a CD-19 that works in paraffin.  The way to go seems to be
> CD-79a.  I'll give that a try and see if the pathologists will be satisfied.
> Thanks again
> 
> Joe Nocito, B.S., HT(ASCP)QIHC
> Histology Supervisor
> Christus Santa Rosa Hospitals
> San Antonio, Texas
> 
> ----------------------------------------------------------------------
> 
> Date: 27 Mar 1999 10:30:33 -0600
> From: "D. Hammer" <hammerd@u.washington.edu>
> Subject: Correction on Medicare Billing
> 
> Histonetters,
> 
> A couple of months ago, in response to a discussion on Medicare Billing, I
> posted our way of handling the 72 hour rule when receiving specimens from
> outside clients.  (I stated to comply with the Rules we were going to have
> to bill the institution for the Technical Component on these as the
> payment is included in the DRG paid to the orriginating Hospital.)
> 
> More recent information (an overlooked Rule) has cleared the air.  When
> the DRG's were designed, for some reason Anatomical Pathology fees were
> not included. Therefore Medicare may be billed for the Technical Component
> by the institution performing the work.  The Professional Fee has always
> been understood to be billed to Medicare.
> 
> This really lifts a burden off the billing process for both the submitting
> and performing institutions.
> 
> I sure hope the APG's (APC's) will be set up in the same way for Out
> Patients or we may have to bill differently when they are in place. :(
> 
> Don
> PS  I am at home and do not have the Reference # but will send it out on
> Monday.
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Don Hammer, Administrative Director            UNIVERSITY OF WASHINGTON
> Hospital Pathology, Box 356100                     MEDICAL CENTER
> 1995 NE Pacific St.
> Seattle Washington, 98195                  ~Where Knowledge Comes To Life~
> (206) 548-6401 Fax: (206) 548-4928
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> 
> ----------------------------------------------------------------------
> 
> Date: 27 Mar 1999 11:45:41 -0600
> From: "Douglas A. Porter" <porter.doug@acd.net>
> Subject: Histonet Archive
> 
> Marvin Hanna wrote:
> 
> Hi Histonetters,
> We have been running a search engine for pathologists and laboratory
> professionals for the past 6 months at http://www.pathsearch.com. A few of
> my friends and co-subscribers on Histonet  have asked if we could archive
> Histonet, so...
> There is now an archive of Histonet messages since October, 1998 on the web
> server at http://www.pathsearch.com/histonet.html. They are available as
> either an archive by dates or searchable by the search engine. The web
> server updates the archive hourly.
> We have also searched and indexed a number of other histology resources on
> the Internet and provided a specialized search engine for them and Histonet.
> You can search sites either collectively or individually at
> http://www.pathsearch.com/histosearch.html. Histosearch is a subset of over
> 10, 000 pages that is included in the over 50,000 pages searched and indexed
> on Pathsearch.
> We have also set up a 24 hour a day chat server, the ability to publish home
> pages or technical papers on the Internet in a couple of minutes, and
> intelligent agent technology for both Pathsearch and Histosearch.
> The Histonet archives, Histosearch, and Pathsearch are free to histologists,
> pathologists, and laboratory professionals on the Internet.  We do plan on
> offering sponsorships on Histosearch and Pathsearch to help cover the cost
> of colocating the server on a T-3 line, hardware, software and time.
> Linda and Herb have done a great service for the field of histology in
> setting up and running Histonet. I hope archiving this information will
> benefit the participants of Histonet.
> Marvin Hanna
> webmaster@pathsearch.com
> 
> Marvin,
> 
> I have been archiving selected Histonet emails since November of 1997.  I
> have 458 to date!  If you would like to somehow add those to yours I would
> be happy to assist in any way I can.  Thanks and good luck!
> 
> Doug
> porter.doug@acd.net
> 
> ----------------------------------------------------------------------
> 
> Date: 27 Mar 1999 11:55:42 -0600
> From: "Alexander Nader" <Alexander.Nader@univie.ac.at>
> Subject: Re: CD-19
> 
> On Sat, 27 Mar 1999 09:51:01 -0600, Nocito, Joseph wrote:
> 
> >Thanks to those who responded to my CD-19 question.  I told my pathologists
> >that there wasn't a CD-19 that works in paraffin.  The way to go seems to
> be
> >CD-79a.  I'll give that a try and see if the pathologists will be
> satisfied.
> >Thanks again
> 
> Be carefull with CD79a: I've seen a couple of T-cell lymphomas (mostly
> peripheral large
> cell variant) staining very nicely with CD79a.
> 
>                         Alexander Nader
>                         Alexander.Nader@univie.ac.at
>                         HTTP://members.teleweb.at/anader
> 
> ----------------------------------------------------------------------
> 
> Date: 27 Mar 1999 11:56:07 -0600
> From: "Alexander Nader" <Alexander.Nader@univie.ac.at>
> Subject: Re: AUA1
> 
> On Fri, 26 Mar 1999 11:37:40 -0800, Technical Services wrote:
> 
> >Hi Dave,
> >This is listed as a neoplastic epithelial marker, and I show that
> Bionostics
> >has it, their number is 508-263-3856. I also have a listing for a
> >Bionostics, Inc and in case this is the company you need, their number is
> >416-789-3936.
> >
> 
> Does anybody know if this antibody is also sold in Europe?
> 
>                         Alexander Nader
>                         Alexander.Nader@univie.ac.at
>                         HTTP://members.teleweb.at/anader
> 
> ----------------------------------------------------------------------
> 
> Date: 27 Mar 1999 13:15:21 -0600
> From: "Hewlett Bryan  (CMH)" <HEWLETT@EXCHANGE1.CMH.ON.CA>
> Subject: RE: CD-19
> 
> Alexander,
> 
> Is that anecdotal or a published observation?
> We have only seen anomalous reactions with CD79a on a couple of
> occasions, both times with epithelia!
> But then again, it's possible to stain anything with anything if you
> really try hard!!
> 
> Bryan
> 
> >----------
> >From:  Alexander Nader[SMTP:Alexander.Nader@univie.ac.at]
> >Sent:  March 27, 1999 12:45 PM
> >To:    Histonet; Nocito, Joseph
> >Subject:       Re: CD-19
> >
> >On Sat, 27 Mar 1999 09:51:01 -0600, Nocito, Joseph wrote:
> >
> >>Thanks to those who responded to my CD-19 question.  I told my
> pathologists
> >>that there wasn't a CD-19 that works in paraffin.  The way to go seems to
> be
> >>CD-79a.  I'll give that a try and see if the pathologists will be
> satisfied.
> >>Thanks again
> >
> >Be carefull with CD79a: I've seen a couple of T-cell lymphomas (mostly
> >peripheral large
> >cell variant) staining very nicely with CD79a.
> >
> >
> >                       Alexander Nader
> >                       Alexander.Nader@univie.ac.at
> >                       HTTP://members.teleweb.at/anader
> >
> >
> >
> 
> ----------------------------------------------------------------------
> 
> Date: 27 Mar 1999 22:45:42 -0600
> From: "Masayuki Miyagishima, MD" <mmiyagis+@pitt.edu>
> Subject: mice frozen tissue IHC protocol.
> 
> This is a very fundamental question.
> What is the best or standard IHC protocol for FROZEN MICE heart and
> vessel?
> They are 4% paraformaldehyde perfusion fixed and sit for 2 hours in ice
> cold 4 % paraformaldehyde and then treated with 30 % sucrose for
> overnight, then embedded in OCT and frozen in Isopentan.  The tissue is
> limited and small, but we need to do many IHCs.
> 
> 1) When we cut the frozen tissue samples, we cut about 50 sections at
> once and put them into -70 fridge for the future IHCs.  Do you think I
> should refix them and air-dried before putting them into -70?
> 
> 2) After taking out from -70 fridge, we treat the sections with 10% Goat
> serum, then treat with rabbit primary antibody for 2 hours at RT, then
> with goat anti-rabbit secondary antibody for 2 hours at RT.  Do you have
> any modifications for mice tissue?
> 
> Thank you in advance.
> 
> - --
> Masayuki Miyagishima, MD
> University of Pittsburgh,
> Department of Surgery
> 412-647-2345, and ask the hospital operator to page 3228, please.
> 
> Here are the messages received yesterday!
 
Hi Melissa, 
What type of information are you looking for in doing IHC on skin? We do
IHC work on skins almost daily and don't treat them differently from
other surgical cases.
Katri