>From: "Beth Bray" <bbray@netside.com>
>To: "Jill McVee" <jm36@st-andrews.ac.uk>, <histonet@pathology.swmed.edu>
>Subject: Re: Carbonic anhydrase
>Date: Tue, 23 Mar 1999 21:36:03 -0500
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>Dear Jill,
>
>For my senior year research, I was tracking the location of carbonic
>anhydrase during sclerite formation in a species of octocoral found here
>along the East and Gulf coasts of the US.  I used rabbit antibodies made
>against the CA of our little "critter" and then labeled with gold-tagged
>goat anti-rabbit antibodies.  Then I post-stained with osmium.  The TEM
>images were lovely after the post-staining.  They looked just like they had
>been prepared for structural observation rather than antibody labeling.  I
>had quite a time with high backgrounds at first, but was able to solve that
>problem by using Tween 20 and immunoprecipitating my primary antibody using
>Staph A.  If any of this sounds interesting to you, let me know and I will
>send you what I have.
>
>Regards,
>Beth Bray
>bbray@netside.com
>