Re: Capillary gap technology

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From:Katie B <>
To:"Dr. Naseem" <>, HistoNet <>
Date:Mon, 22 Mar 1999 14:44:06 -0800 (PST)
Content-Type:text/plain; charset=us-ascii

---"Dr. Naseem" <> wrote:
>   I'll be grateful if someone can tell me about "CAPILLARY GAP
> of immunohistochemistry
> Dr. Naseem

I use the Fisher MicroProbe Staining System for doing this type of
IHC.  The main advantage is that the volumes of reagents used is
greatly reduced as only ~150ul is needed of your antibodies to stain
two slides.   So although you have to spend the money to buy the
staining station and the special Probe-on-Plus slides, it goes so much
faster than doing it the old fashioned way (dropping the reagents onto
slides laying horizontally) and you save on reagent costs.  I think
it's the greatest thing for staining large batches of slides.

You have to cut your histo sections onto the special Probe-on-Plus
slides.  These are designed so that when you put two of these slides
face-to-face there is a gap between the two slides where the tissues
are.  This gap is just far enough apart that when you put a corner of
this slide sandwich into a drop of your reagents, the solution gets
sucked up the gap by capillary action.  You can remove the solution by
blotting it out onto paper towels.

I think the Fisher catalog has a nice picture of the system they sell
and I'm sure they would send you detailed info on how it works if you
call them.  

Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

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