---"Dr. Naseem" <nasimas@khi.compol.com> wrote:
>
> HeLLO FELLOWS,
>   I'll be grateful if someone can tell me about "CAPILLARY GAP
TECHNOLOGY"
> of immunohistochemistry
> THANX
> Dr. Naseem

I use the Fisher MicroProbe Staining System for doing this type of
IHC.  The main advantage is that the volumes of reagents used is
greatly reduced as only ~150ul is needed of your antibodies to stain
two slides.   So although you have to spend the money to buy the
staining station and the special Probe-on-Plus slides, it goes so much
faster than doing it the old fashioned way (dropping the reagents onto
slides laying horizontally) and you save on reagent costs.  I think
it's the greatest thing for staining large batches of slides.

You have to cut your histo sections onto the special Probe-on-Plus
slides.  These are designed so that when you put two of these slides
face-to-face there is a gap between the two slides where the tissues
are.  This gap is just far enough apart that when you put a corner of
this slide sandwich into a drop of your reagents, the solution gets
sucked up the gap by capillary action.  You can remove the solution by
blotting it out onto paper towels.

I think the Fisher catalog has a nice picture of the system they sell
and I'm sure they would send you detailed info on how it works if you
call them.  


 
==
Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

*new* e-mail: bresee98@yahoo.com

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