Re: Bad Biopsy Cassettes/mesh cassettes.
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From: | "R.Wadley" <s9803537@pop3.unsw.edu.au> |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Wed, 24 Mar 1999 10:05:46 +1000 |
Content-Type: | text/plain; charset="us-ascii" |
Dear Katie,
In a lab I worked in we had problems with floating cassettes, principally
those with sponges in. The problem was solved by keeping the sponges moist
in 20% ethanol. This lowers the surface tension of the formalin etc, &
increases the wettability of the sponge. I assume this principle would
hold true for these fine mesh cassettes. So place specimen in cassette dip
in 20% ethanol (or what ever works for you) then into the processor rack.
Once there full of fluid & being processed there should be no problem.
Hope this helps.
Regards
Rob W.
At 02:08 PM 3/22/99 -0800, you wrote:
>I too have been having problems with biopsy cassettes not infiltrating
>well. I have been using Fisher's Tissue Path Microsette Biopsy
>Cassettes and my histo lab informs me that sometimes they don't
>infiltrate well and there are "floater" cassettes. I only like this
>kind of cassette because it has 6 little compartments in the cassette
>which is great for keeping small mouse tissues organized and less
>likely to flip over from the desired cut face. The size of the
>infiltration pores is irrelevant to me as the tissues are large enough
>not to get lost. Previously, I had been cutting little holes in
>sponges to stabilize the tissues while using normal cassettes
>(annoyingly time consuming!)
>
>So my question is: does any one know of a supplier of cassettes with
>normal sized infiltration holes, but with these cute little
>compartments inside?
>==
>Catherine "Katie" Bresee Bennett
>Laboratory for Experimental Pathology
>Department of Veterinary Pathology
>Michigan State University
>
>*new* e-mail: bresee98@yahoo.com
>
>_________________________________________________________
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>
>
>
R. Wadley, B.App.Sc, M.L.S
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) +61 (2) 9385 3517
Ph (AH) +61 (2) 9555 1239
Fax +61 (2) 9385 1591
E-mail r.wadley@unsw.edu.au
www http://www.unsw.edu.au/clients/microbiology/CAF.html
(Under development)
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