Re: ANTIGEN UNMASKING

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From:Tim Morken <timcdc@hotmail.com>
To:histonet@Pathology.swmed.edu
Reply-To:
Date:Thu, 01 Apr 1999 09:10:25 -0500 (EST)
Content-Type:text/plain

Mr. richards,

Yes, we have done this and it works much better than any other heat 
retreival method. We have a Singer stream iron with 15 heat settings, 
but probably any would do. We got it at Wal-Mart. Of course you need a 
suitable surface to do the ironing and we found one in the bin out back 
- yes, a piece of cardboard is the best, and is perfectly recycleable. 
We generally iron for 6 minutes at setting 12, wool, I believe. One 
great benefit, besides the antigen unmasking, is the elimation of 
wrinkles.

As an aside, we have an entire "kitchen" set up now, what with out 
microwave, steamer and pressure cooker. We are also using a Sears 
Hotpoint clothes dryer to dry slides. much cheaper than those sold in 
those expensive lab supply catalogs! We did have to disable the tumble 
function, however, as our slides got all mixed up and had to be 
re-sorted!


Tim Morken, B.A., EMT(MSA), HTL(ASCP) 
Infectious Disease Pathology
Centers for Cookery Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

FAX:  (404)639-3043



----Original Message Follows----
From: "M.  Richards." <REE3@leicester.ac.uk>
To: histonet@pathology.swmed.edu
Subject: Re: ANTIGEN  UNMASKING
Date: Thu, 01 Apr 1999 08:31:00 +0100 (BST)

   Philips et al in a recent  publication(J. Appl. Dom.  Sci.  
Tech.1999;22,37-44) 
described  the  use of  the  domestic  steam  iron  to  achieve  antigen  
unmasking in paraffin  sections.The distilled water being  replaced  by 
citrate  
buffer  pH6, and  by 'ironing' the  sections  through absorbent  brown  
paper 
the  dewaxing  step  is  combined  with the  antigen  unmasking.
                      I  would  be  interested to  hear  from anyone  
who has had  
any  success with this  technique.
                                           Thanks  in  advance

                                                M.  Richards........




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