Re: ANTIGEN UNMASKING

<< Previous Message | Next Message >>
From:"M. Richards." <REE3@leicester.ac.uk>
To:histonet@Pathology.swmed.edu
Reply-To:
Date:Thu, 01 Apr 1999 08:31:00 +0100 (BST)
Content-Type:

       

   Philips et al in a recent  publication(J. Appl. Dom.  Sci.  Tech.1999;22,37-44) 
described  the  use of  the  domestic  steam  iron  to  achieve  antigen  
unmasking in paraffin  sections.The distilled water being  replaced  by citrate  
buffer  pH6, and  by 'ironing' the  sections  through absorbent  brown  paper 
the  dewaxing  step  is  combined  with the  antigen  unmasking.
                      I  would  be  interested to  hear  from anyone  who has had  
any  success with this  technique.
                                           Thanks  in  advance

                                                M.  Richards........




<< Previous Message | Next Message >>