Re: ANTIGEN UNMASKING
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From: | "M. Richards." <REE3@leicester.ac.uk> |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Thu, 01 Apr 1999 08:31:00 +0100 (BST) |
Content-Type: | |
Philips et al in a recent publication(J. Appl. Dom. Sci. Tech.1999;22,37-44)
described the use of the domestic steam iron to achieve antigen
unmasking in paraffin sections.The distilled water being replaced by citrate
buffer pH6, and by 'ironing' the sections through absorbent brown paper
the dewaxing step is combined with the antigen unmasking.
I would be interested to hear from anyone who has had
any success with this technique.
Thanks in advance
M. Richards........
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