RE: cryo sample - problem
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From: | Cynthia Favara <cfavara@niaid.nih.gov> |
To: | "'Kelly Randall'" <kellyr@neuro.hfh.edu>, HistoNet@Pathology.swmed.edu |
Reply-To: | |
Date: | Mon, 29 Mar 1999 18:42:04 -0500 |
Content-Type: | text/plain; charset="iso-8859-1" |
Kelly,
Is it necessary to freeze the cells before spinning?
Cynthia
-----Original Message-----
From: Kelly Randall [mailto:kellyr@neuro.hfh.edu]
Sent: Monday, March 29, 1999 1:43 PM
To: HistoNet@Pathology.swmed.edu
Subject: cryo sample - problem
We are trying to cryosection samples of brain cells that have been spun
down in a centrifuge tube. The frozen pellets are "popped" out of
the tube, but they begin to thaw when the embedding media is added.
If we tried to use a thinner walled centrifuge tube would it be possible
to section the pellets AND the tube together? We have a cryotome
that used disposable blade, and another that has a stainless blade. I
guess that this is quite possible right, since many of you cut bone, but
would like some tips if anyone has them to offer.
Thanks in advance!
Kelly
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