RE: cryo sample - problem

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From:Cynthia Favara <>
To:"'Kelly Randall'" <>,
Date:Mon, 29 Mar 1999 18:42:04 -0500
Content-Type:text/plain; charset="iso-8859-1"

	Is it necessary to freeze the cells before spinning?

-----Original Message-----
From: Kelly Randall []
Sent: Monday, March 29, 1999 1:43 PM
Subject: cryo sample - problem

We are trying to cryosection samples of brain cells that have been spun 
down in a centrifuge tube.   The frozen pellets are "popped" out of 
the tube, but they begin to thaw when the embedding media is added.
If we tried to use a thinner walled centrifuge tube would it be possible 
to section the pellets AND the tube together?  We have a  cryotome 
that used disposable blade, and another that has a stainless blade.  I 
guess that this is quite possible right, since many of you cut bone, but 
would like some tips if anyone has them to offer.

Thanks in advance!

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