RE: cryo sample - problem

<< Previous Message | Next Message >>
From:Cynthia Favara <cfavara@niaid.nih.gov>
To:"'Kelly Randall'" <kellyr@neuro.hfh.edu>, HistoNet@Pathology.swmed.edu
Reply-To:
Date:Mon, 29 Mar 1999 18:42:04 -0500
Content-Type:text/plain; charset="iso-8859-1"

Kelly,
	Is it necessary to freeze the cells before spinning?
Cynthia

-----Original Message-----
From: Kelly Randall [mailto:kellyr@neuro.hfh.edu]
Sent: Monday, March 29, 1999 1:43 PM
To: HistoNet@Pathology.swmed.edu
Subject: cryo sample - problem



We are trying to cryosection samples of brain cells that have been spun 
down in a centrifuge tube.   The frozen pellets are "popped" out of 
the tube, but they begin to thaw when the embedding media is added.
If we tried to use a thinner walled centrifuge tube would it be possible 
to section the pellets AND the tube together?  We have a  cryotome 
that used disposable blade, and another that has a stainless blade.  I 
guess that this is quite possible right, since many of you cut bone, but 
would like some tips if anyone has them to offer.

Thanks in advance!
Kelly  



<< Previous Message | Next Message >>