HI, Marsha.

We use a Black and Decker steamer  (20 Min) for all of our antibodies
except for ER and PR. For these we have gone to a water bath at 80
degrees for 1.5 -2 hrs. because of tissue adhesion issues. Our breast
tissues just love to fall off!

Diana Goodwin, HT
Trenton,  NJ
> ----------
> From: 	marsha r price[SMTP:mprice26@juno.com]
> Sent: 	Wednesday, March 24, 1999 5:52 PM
> To: 	chark1@gte.net
> Cc: 	ALF569@aol.com; histonet@Pathology.swmed.edu
> Subject: 	Re: auto-ihc stainers
> 
> Hi Histonetters,
> I would like to know how many of you if any are using a vegetable
> steamer
> for Antigen Retrieval. Thank you for your replies.
> Marsha Price HT (ASCP), QIHC (ASCP)
>