I use IGF1 and 2 from Intergen, works well on paraffin processed aldehyde
fiixed tissue with heir method.  I use a pressure cooker in a MW in citrate
buff. PH 6 for 18 min. on high power, 750 watt MW.
Patsy Ruegg

		-----Original Message-----
		From:	Mary Ann Miller [mailto:millerma@email.uc.edu]
		Sent:	Wednesday, March 17, 1999 3:20 PM
		To:	HistoNet@Pathology.swmed.edu
		Subject:	antibodies

		Dear Histonet Friends,

		I have spent the afternoon searching Linscott's and the web
for antibodies
		to Insulin Growth Factor 1 and 2. There are many many of
both, but I need
		to use them in human formalin fixed paraffin embedded tissue
to answer a
		reviewer's questions before we can get a paper published.
Does anyone know
		of ones that work reliably?

		Thanks for any information.    Mary Ann

		Mary Ann Miller
		University of Cincinnati
		College of Medicine  ML 529
		231 Bethesda Avenue
		Cincinnati, Ohio  45267
		FAX: (513) 558-2289