RE: Wavy Boney Tissues
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From: | "Montague, Donna C" <MontagueDonnaC@exchange.uams.edu> |
To: | "'Katie B'" <bresee98@yahoo.com> |
Reply-To: | |
Date: | Fri, 26 Mar 1999 13:01:37 -0600 |
Content-Type: | text/plain; charset="iso-8859-1" |
Katie:
At what thickness were they cut, 4 - 6 microns or thicker? Did you check the
end-point of decalcification with 5 %ammonium oxalate solution? What type of
paraffin do you use to emded the tissues? Were they cut using a steel knife
or a disposable blade? What is the temperature of your slide warming
station?
All these factors can effect the quality of your bony sections. Donna
Montague, UAMS Orthopaedic Research
-----Original Message-----
From: Katie B [mailto:bresee98@yahoo.com]
Sent: Friday, March 26, 1999 9:43 AM
To: Histonet Server
Subject: Wavy Boney Tissues
I have sections that are of cross sections of rat heads
and a recent batch came back with the tissues all wavy
on the slide. They were fixed in Zinc Formalin (4d)
then decalcified in 13% Formic Acid for 4d. The bone
is all intact in the section (no shattering, cutting
was easy) so I think the tissues were adequately
decalcified. However, our protocol calls for a 4 to 7
day decal, and these rat heads were a bit on the larger
side, so I'm wondering if the tissues were just
slightly under decalcified. Any suggestions I can pass
onto our histo lab to help them achieve flat
sections??
===
Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University
*new* e-mail: bresee98@yahoo.com
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