Carrie,
	We do histochemistry on animal tissue here and our protocol calls
for 12-24hr in NBF aiming for 12hr. at that time we place the specimen in
PBS @ 4C. I make every effort to process within 24 hours but have left it in
PBS up to 72hrs and haven't had difficulty as long as the tissue is fixed.

Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov


> ----------
> From: 	Schray, Carrie[SMTP:Carrie.Schray@wl.com]
> Sent: 	Tuesday, March 16, 1999 8:09 AM
> To: 	'vandeplas@aurion.nl'
> Cc: 	'Histonet@Pathology.swmed.edu'
> Subject: 	Tissue storage
> 
> I saw on the Histonet your response to Ian Montgomery about tissue
> storage.
> Sometimes we have studies that require Immunohistochemistry or In-situ on
> tissues (animal) later in the process.  We have been fixing our tissues
> for
> no longer that a week in 10%NBF, and then transferring them into 60%
> Ethanol.  Usually, depending on our workload they only are in alcohol for
> a
> few days, but this has given us problems with certain tissues and really
> dries them out. Would you have any suggestions about something other than
> 60% Ethanol to store them in after fixation, or would your PBS solution
> work?  How much H2 gas is formed with that?  We store our tissues in
> Bitran
> bags (like a ziplock), and I wondered what, if anything, problems may
> occur.
> Carrie Schray  HT(ASCP)
> Parke-Davis Pharmaceuticals
> Ann Arbor, Michigan
>