RE: Thanks and another question

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From:Patsy.Ruegg@UCHSC.edu
To:angelrj@email.uc.edu
Reply-To:
Date:Wed, 24 Mar 1999 09:39:00 -0700
Content-Type:text/plain; charset="iso-8859-1"

I get Sirrus Red listed as "Siriusrot F3BA  1A 280 from a German outfit
called Chroma-Gesellschaft  Schmid GmbH+Co. D-7316 Konegen/N.  it is
distributed by Roboz Surg in Connecicut
Patsy Ruegg

		-----Original Message-----
		From:	Rita [mailto:angelrj@email.uc.edu]
		Sent:	Wednesday, March 24, 1999 6:37 AM
		To:	histonet@Pathology.swmed.edu
		Subject:	Thanks and another question

		Thanks for all the replies to my question about the Sirius
Red stain. 
		However, I did get conflicting answers of whether it shows
the green
		birefringence.  One person stated that the Sirius Red does
not stain
		amyloid, but my procedure in my AFIP Lab. Methods in
Histotechnology
		(1992) does state that it stains amyloid.  

			My next question is:  The only Sirius Red I could
find in a catalogue
		is Direct Red 80 from Aldrich.  (Sirius Red is in
parenthesis)  My
		procedure calls for Sirius Red F3BA.  Is this the same
thing?  Also the
		dye content of the Direct Red 80 is ~30%.  Does this have
any bearing on
		the matter?  And also does anyone know where I can order
Sirius Red
		F3BA?  I would greatly appreciate help on this matter.
Thank you all
		for the help I've received so far.

			Sincerely,

			Rita Angel



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