RE: Glycogen Phosphorylase

<< Previous Message | Next Message >>
From:"Kellar, Eric" <kellarec@MSX.UPMC.EDU>
To:"'Coskran, Timothy M '" <timothy_m_coskran@groton.pfizer.com>, "''Histonet@pathology.swmed.edu' '" <Histonet@Pathology.swmed.edu>
Reply-To:
Date:Mon, 29 Mar 1999 21:37:49 -0500
Content-Type:text/plain; charset="iso-8859-1"

This online enzyme histochemical technique I have tried, it stains
blue/black and uses rabbit muscle glycogen.

http://www.ccc.nottingham.ac.uk/~mpzjlowe/protocols/phosphor.html

 Copyright 1997 University of Nottingham Medical School Division of
Histopathology.   


Eric Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center


-----Original Message-----
From: Coskran, Timothy M
To: 'Histonet@pathology.swmed.edu'
Sent: 3/29/99 8:58 AM
Subject: Glycogen Phsphorylase

Hello,

Does anyone have any advice, suggestions, etc on trying to localize
glycogen
phosphorylase activity by using IHC methods?  All of my initial runs
have a
great deal of background.  Avidin-biotin blocks, serum blocks have all
been
used.  Does anyone know of an enzyme histochemical method for the
identification of glycogen phosphorylase?

Thanks 


Tim Coskran
Pfizer



<< Previous Message | Next Message >>