RE: Glycogen Phosphorylase
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From: | "Kellar, Eric" <kellarec@MSX.UPMC.EDU> |
To: | "'Coskran, Timothy M '" <timothy_m_coskran@groton.pfizer.com>, "''Histonet@pathology.swmed.edu' '" <Histonet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Mon, 29 Mar 1999 21:37:49 -0500 |
Content-Type: | text/plain; charset="iso-8859-1" |
This online enzyme histochemical technique I have tried, it stains
blue/black and uses rabbit muscle glycogen.
http://www.ccc.nottingham.ac.uk/~mpzjlowe/protocols/phosphor.html
© Copyright 1997 University of Nottingham Medical School Division of
Histopathology.
Eric Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center
-----Original Message-----
From: Coskran, Timothy M
To: 'Histonet@pathology.swmed.edu'
Sent: 3/29/99 8:58 AM
Subject: Glycogen Phsphorylase
Hello,
Does anyone have any advice, suggestions, etc on trying to localize
glycogen
phosphorylase activity by using IHC methods? All of my initial runs
have a
great deal of background. Avidin-biotin blocks, serum blocks have all
been
used. Does anyone know of an enzyme histochemical method for the
identification of glycogen phosphorylase?
Thanks
Tim Coskran
Pfizer
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