Hi,
	Frozen lymphoid tissues after cryo-sectioning at -20 C and staining
features some
debris (hair like, glass like structures and other junks). It is very
annoying when you have it sitting right at the center of a picture perfect
follicle. Does any one have an idea of the source of these debris?  I think
that this occurs, during sectioning but the source of these debris I have
not been able to determine.
	Again, thanks to all of you that gave suggestions on methods of
freezing lymphoid tissues. I have recently made excellent sections (cracks
and holes free) from a rabbit appendix tissue frozen in OCT placed in a
mixture of isopentane and dry ice.
Harold.