Junks on Stained (Frozen Lymphoid) Tissue

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From:Harold Obiakor <HOBIAKOR@niaid.nih.gov>
To:"'histonet@pathology.swmed.edu'" <histonet@Pathology.swmed.edu>
Date:Fri, 19 Mar 1999 12:23:29 -0500

	Frozen lymphoid tissues after cryo-sectioning at -20 C and staining
features some
debris (hair like, glass like structures and other junks). It is very
annoying when you have it sitting right at the center of a picture perfect
follicle. Does any one have an idea of the source of these debris?  I think
that this occurs, during sectioning but the source of these debris I have
not been able to determine.
	Again, thanks to all of you that gave suggestions on methods of
freezing lymphoid tissues. I have recently made excellent sections (cracks
and holes free) from a rabbit appendix tissue frozen in OCT placed in a
mixture of isopentane and dry ice.

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