Junks on Stained (Frozen Lymphoid) Tissue

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From:Harold Obiakor <HOBIAKOR@niaid.nih.gov>
To:"'HistoNet Server'" <HistoNet@Pathology.swmed.edu>
Date:Thu, 18 Mar 1999 19:16:18 -0500
Content-Type:text/plain; charset="iso-8859-1"

	Frozen lymphoid tissues after sectioning and staining features some
debris (hair like, glass like structures and other junks). It is very
annoying when you have it sitting right at the center of a picture perfect
follicle. Does any one have an idea of the source of these debris?  I think
that this occurs, during sectioning but the source of these debris I have
not been able to determine.
	Again, thanks to all of you that gave suggestions on methods of
freezing lymphoid tissues. I have recently made excellent sections (cracks
and holes free) from a rabbit appendix tissue frozen in OCT placed in a
mixture of isopentane and dry ice.

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