[Fwd: corornary artery plaque]
<< Previous Message | Next Message >>
From: | Donna Carr <dkc@odsgc.net> |
To: | "'Histonet'" <Histonet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Fri, 26 Mar 1999 09:39:09 -0600 |
Content-Type: | text/plain; charset=us-ascii |
Gayle
We normally decal all coronary arteries that contain plaque. Before embedding.
It doesn't always work 100%. But there is always the option of decaling the block
face as you suggested. I can't imagine trying to do frozen sections on arteries
with plaque. That sounds like a nightmare to me.
Donna
Gayle Callis wrote:
> It's been a long time, but do most of your decalcify coronary arteries
> routinely when they contain plaque? We have a dicey project using human
> coronary arteries, initially setup to use frozen sections - with
> lousy success, aka sections! even with our Instrumedics) Now trying
> a D profile tungsten carbide with tape transfer, the c profile TC was not
> totally successful, so new knife and temp tweaking. Finding the plaque
> area does not like the tape, nor the knife edge, scrunched areas prevail.
> It is a long, complicated story on what/how/why all this is being done.
>
> I suggested they do paraffin sections to get better morphology of both
> artery and plaque, with better sectioning hence the decalcification question.
> I may try a surface decalcification on block face, not a favorite,
> but thought some outside input from the clinical experts would be nice.
>
> A tutorial on plaque will also be appreciated, as I said it's been a long time
> since I had to deal with these specimens, and have been trying to recall
> composition of layers (fibrin, lipids?, sometimes calcium, etc).
>
>
> Gayle Callis
> Veterinary Molecular Biology
> Montana State University
> Bozeman MT 59717-3610
>
<< Previous Message | Next Message >>