[Fwd: corornary artery plaque]

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From:Donna Carr <dkc@odsgc.net>
To:"'Histonet'" <Histonet@Pathology.swmed.edu>
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Date:Fri, 26 Mar 1999 09:39:09 -0600
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Gayle
We normally decal all coronary arteries that contain plaque.  Before embedding.
It doesn't always work 100%. But there is always the option of decaling the block
face as you suggested.  I can't imagine trying to do frozen sections on arteries
with plaque.  That sounds like a nightmare to me.
   Donna

Gayle Callis wrote:

> It's been a long time, but do most of your decalcify coronary arteries
> routinely when they contain plaque?  We have a dicey project using human
> coronary arteries, initially setup to use frozen sections - with
> lousy success, aka sections! even with our Instrumedics)  Now trying
> a D profile tungsten carbide with tape transfer, the c profile TC was not
> totally successful, so new knife and temp tweaking. Finding the plaque
> area does not like the tape, nor the knife edge, scrunched areas prevail.
> It is a long, complicated story on what/how/why all this is being done.
>
> I suggested they do paraffin sections to get better morphology of both
> artery and plaque, with better sectioning hence the decalcification question.
> I may try a surface decalcification on block face, not a favorite,
> but thought some outside input from the clinical experts would be nice.
>
> A tutorial on plaque will also be appreciated, as I said it's been a long time
> since I had to deal with these specimens, and have been trying to recall
> composition of layers (fibrin, lipids?, sometimes calcium, etc).
>
>
> Gayle Callis
> Veterinary Molecular Biology
> Montana State University
> Bozeman MT 59717-3610
>






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