FW: paraformaldehyde
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From: | "Celebre, Gino" <celebre@HAMCIVHOS.ON.CA> |
To: | "'histonet@pathology.swmed.edu'" <histonet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Thu, 18 Mar 1999 09:57:01 -0500 |
Content-Type: | text/plain; charset="us-ascii" |
>----------
>From: Celebre, Gino
>Sent: Thursday, March 18, 1999 9:18 AM
>To: 'histonet@pathology.swmed.edu'
>Subject: paraformaldehyde
>
>I used to use paraformaldehyde for flowcytometry applications and found
>that using fresh (daily) paraform. gave us the best fixation, but we
>kept it for a week.
>
>I agree that you should not store for more than a week if necessary
>(turns basically to water and is useless)
>
>However, I did keep a stock paraformaldehyde solution for up to a month.
>I made a 10% paraformaldehyde solution in PBS. Heated it to 80degrees C
>and no higher, let it cool and filtered it. It was remade each month.
>
>My working solution was a 0.5% solution made with the stock and ultra
>pure PBS. We kept it in the fridge for no more than a week.
>
>Gino Celebre, MLT
>Department of Pathology and Molecular Medicine
>Hamilton Health Sciences Corporation
>Hamilton, Ontario, Canada
>(905)527-4322 ext. 6145
>celebre@hamcivhos.on.ca
>
>
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