FW: paraformaldehyde

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From:"Celebre, Gino" <celebre@HAMCIVHOS.ON.CA>
To:"'histonet@pathology.swmed.edu'" <histonet@Pathology.swmed.edu>
Date:Thu, 18 Mar 1999 09:57:01 -0500
Content-Type:text/plain; charset="us-ascii"

>From: 	Celebre, Gino
>Sent: 	Thursday, March 18, 1999 9:18 AM
>To: 	'histonet@pathology.swmed.edu'
>Subject: 	paraformaldehyde
>I used to use paraformaldehyde for flowcytometry applications and found
>that using fresh (daily) paraform. gave us the best fixation, but we
>kept it for a week.
>I agree that you should not store for more than a week if necessary
>(turns basically to water and is useless)
>However, I did keep a stock paraformaldehyde solution for up to a month.
>I made a 10% paraformaldehyde solution in PBS. Heated it to 80degrees C
>and no higher, let it cool and filtered it. It was remade each month.
>My working solution was a 0.5% solution made with the stock and ultra
>pure PBS. We kept it in the fridge for no more than a week. 
>Gino Celebre, MLT
>Department of Pathology and Molecular Medicine
>Hamilton Health Sciences Corporation
>Hamilton, Ontario, Canada
>(905)527-4322 ext. 6145

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