Carbonic anhydrase

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From:Ian Montgomery <>
Date:Wed, 24 Mar 1999 11:46:16 +0000
Content-Type:text/plain; charset="us-ascii"

>Date: Tue, 23 Mar 1999 12:56:56 +0000 (GMT)
>From: (Jill McVee)
>Subject: Carbonic anhydrase
>MIME-version: 1.0
> Dear Histo netters,
>                     I wonder if you can help?I,m need a  method for
>demonstrating carbonic anhydrase. I have looked in Pearse but I wonder if
>anyone out there has a more up to date method (Hausler 1958). I am also
>fixing in2.5 % glut, then 30% sucrose and  cutting f/s  .  After staining I
>would like if possible to D.C.M.
>                    Thanks in advance .
>Bute Building
>St.Andrews University

	Run a few trials years ago using a number of techniques,  my
priorities changed so didn't complete the trials. Here are the references
for the techniques I used. They all worked, but would need a wee bit of
tinkering for optimum results.

Brown,D. 1980. Stain Technol. 55. 115-118.
	Carbonic Anhydrase localization in mounted cryostat sections.

Brown,D., Roth,J., Kumpulainen,T. & Orci,L. 1982.
Histochemistry. 1982. 75. 209-213.
	Ultrastructural immunocytochemical localization of carbonic anhydrase.
Presence in intercalated cells of rat collecting tubule.

Sugai,N. & Susumi,I. 1980. J. Histochem. Cytochem. 28. No.6. 511-525.
	Carbonic anhydrase, Ultrastructural localization in the mouse
gastric mucosa and improvemtnes in the technique.

Eichhorn,M. 1988. Histochemistry. 88. 475-479.
	The pesence of carbonic anhydrase in orbital glands. An
enzyme-histochemical study in cynomolgus monkeys and rabbits.

Kazimierczak,J., Sommer,E.W., Philippe,E. & Droz,B. 1986.
Cell Tiss. Res. 245. 487-495.
	Carbonic anhydrase activity in primary sensory neurons.
I. Requirements for the cytochemical localization in the dorsal root
ganglion of chicken and mouse by light and electron microscopy.

Vaananen,H.K. & Autio-Harmainen,H. 1987.
J. Histochem. Cytochem. 35. No.6. 683-686.
	Carbonic anhydrase III: A new histochemical marker for
myoepithelial cells.

	Been wracking the brain for hints and tips but as I didn't spent
much time with the technique can't be of much use. The first reference by
Denis Brown in Stain technology was the one I used most. It's based on the
original method by Hansson, was simple and fairly reliable. If I was to
start again this would be my first trial.
	I'm sure if you run a literature search you'll come up with a bunch
of more recent references.
Best of luck.

Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
G12 8QQ,
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.

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