Aniline blue source?
<< Previous Message | Next Message >>
From: | "R.W.Horobin" <R.W.Horobin@sheffield.ac.uk> |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Fri, 19 Mar 1999 14:17:26 +0000 |
Content-Type: | |
G'day folks
John Baker asked about a source for Aniline blue CI 42780, as used
for the Sterchi's modified Mallory method.
Aniline blue is a trad name for all sorts of mixtures of blue, sulfonated,
triarylmethane dyes. CI 42780 is the CI number for Methyl Blue,
one of the usual components of 'Aniline blue'. You can get MB from
standard suppliers, Sigma and ICN for instance. Or just get a
Stain Commission Certified Aniline blue from anyone, and that should
work OK.
In fact nearly all the 'Aniline blue' and 'Methyl blue' and 'Water
blue' (that's another component in 'Aniline blue' usually) bathces are in
fact mixtures of blue dyes. Whatever it is says on the label, much the
same mix is usually present. A few might be mostly Methyl blue, and
only a single compound. But USUALLY this doesnt matter, as long
as the dye content is OK - hence the recommendation of a Certified
batch.
Bye, Richard
------------------------------------------------------
Richard W Horobin - Dept of Biomedical Science
The University of Sheffield - Sheffield S10 2TN - UK
Dir line: +44-114-222 4667 - Dept fax: +44-114-276 5413
email: 101370.2617@CompuServe.com
<< Previous Message | Next Message >>