trichrome
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From: | Ian Montgomery <ian.montgomery@bio.gla.ac.uk> |
To: | HistoNet@Pathology.swmed.edu |
Reply-To: | |
Date: | Mon, 15 Mar 1999 17:09:47 +0000 |
Content-Type: | text/plain; charset="us-ascii" |
>Date: Mon, 15 Mar 1999 09:31:47 -0700
>From: Katrina Knott <knottk@amc.org>
>Subject: trichrome
>To: histonet <histonet@pathology.swmed.edu>
>MIME-version: 1.0
>
>We have been working on a the Masson's trichrome stain on human uterus. The
>stain looks nice but we are not getting nuclear staining. We are using
>Weigert's Iron Hematoxylin. It seems to look well when we apply it, and
>when we take the tissue out of the stain. Is it possible that we are losing
>staining is subsequent steps due to the acidity, etc? What can be done
>about this? Increase the mordant concentration? Change one of the other
>steps in the procedure? It seemed to stain when we did not use Bouin's to
>post fix... but then the other dyes did not look well.
>
>thanks,
>Katrina
>
Katrina,
Try a Celestine blue/ Haematoxylin sequence.
Ian.
Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Glasgow,
G12 8QQ,
Scotland.
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.
e-mail: ian.montgomery@bio.gla.ac.uk
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