p53 staining in cryosections

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From:Pat Eden <pat.eden@spectrum-ferguson.org> (by way of histonet)
To:histonet <histonet@magicnet.net>
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I am looking for tips on immunohistochemical staining of p53 in frozen
sections of colon tumors.  I am working with an antibody (Sigma
BP53.12)which works well with paraffin sections, however, it doesn't seem
to be as effective with frozen sections.  I am fixing the tissue in cold
acetone for ten minutes, and using a Sigma ExtrAvidin peroxidase DAB
detection system.  Any advice on immunohistochemical protocols on frozen
sections or p53 antibodies would be greatly appreciated.

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