p53 staining in cryosections
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| From: | Pat Eden <pat.eden@spectrum-ferguson.org> (by way of histonet) |
| To: | histonet <histonet@magicnet.net> |
| Reply-To: | |
| Content-Type: | text/plain; charset="us-ascii" |
I am looking for tips on immunohistochemical staining of p53 in frozen
sections of colon tumors. I am working with an antibody (Sigma
BP53.12)which works well with paraffin sections, however, it doesn't seem
to be as effective with frozen sections. I am fixing the tissue in cold
acetone for ten minutes, and using a Sigma ExtrAvidin peroxidase DAB
detection system. Any advice on immunohistochemical protocols on frozen
sections or p53 antibodies would be greatly appreciated.
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