fluorescent nuclear stain

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From:Judy Trogadis <judy@playfair.utoronto.ca> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Dear Histonetters,

We are trying to localize gfp expression in transfected HeLa cells and
would like to use DNA counterstain to distinguish between nuclear and
cytoplasmic distribution. We have tried propidium iodide which I
thought is a nuclear marker, however stained the entire cell. We have
also tried Yo-Pro-3, from Molecular Probes, and it, too, labelled
both the nucleus and the cytoplasm. Our confocal microscope laser does
not have excitation for DAPI.

Any suggestions for another 'red' DNA marker would be highly appreciated.

(And I thought this would be the easy part).


Judy Trogadis
Eye Research Institute and
University of Toronto
Toronto Hospital, Western Div.
399 Bathurst  St.
Toronto, Canada M5T 2S8

phone: 416-603-5088
Fax:   416-603-5126
email: judy@playfair.utoronto.ca

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