Hi all,


I'm just starting to do paraffin embedded sectioning.  There are protocols that
have been set up as standards with our processor, but no one has been able to
explain why they were chosen.  It's been pretty much "because so and so did it
that way."  Since these results will be used in publication, I need to be very
specific about what and how I do thing. What I would like to know is
if anyone would be willing to share their protocol for processing and
deparaffinization of the slide after sectioning.  Also, the theory behind why
you chose that method.  Results?  If you can be specific, that would be great.
If not, that will also be ok.

I will be sectioning mouse heart, muscle, embryo.  Plus
bovine cartilage and human cartilage.  These sections will be stained with
specific antibodies.  Do any of you have suggestion or can site specific
reference that would give me some guidance for fixation of these tissues,
processing methods for paraffin embedding, and how you deparaffinize.  Do
these processes depend on the type of tissue, size, etc., etc.  Also, what
thickness do you usually cut for these types of tissue.

I know this is very general.  Any suggestion would be appreciated.  Thank you.

Noi