bmt decal

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From:Anne van Binsbergen <> (by way of histonet)
To:histonet <>
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Hi there Histonetters,

Our recipe for EDTA decal sol for BMT is as follows:

1750ml dist H20
250g EDTA (disodium salt)

Mix together and neutralise with NH4OH (use litmus paper to check)
(NH+  act as chelating agents too)

After this the solution turns cloudy - this is cleared by continuously
stirring for about 30mins

We decal overnight (after checking for proper fixation - usually 24hrs) and
check manually  - sometimes the tissue is left in the decal fluid for a
further 24hrs - specimens containing cortical bone will take much longer.

We put the decal fluid into the specimen 'pot' - about 50 times the volume
of the tissue

Don't use any metal cassette lids

The tissue does not need to be washed after decal and before processing

Gloves are not necessary as the decal solution is not toxic or corrosive

Our results speak for themselves!!

happy decalling

Annie (in Africa)
Anne S. van Binsbergen
T.I.C. Anatomical Pathology
Chris Hani Baragwanath Hospital
PO Box 1038
Johannesburg 2000
South Africa

tel: 011 4898711
cell: 083 4403343

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