aggressive antigen retrieval

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From:Joyce Kotzuk <> (by way of histonet)
To:histonet <>
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I have problems with section adherence when I try aggressive antigen
retrieval methods such as multiple microwavings, formic acid treatments
etc. I am currently using Vectabond treated slides; I bake the sections at
37 degrees overnight right after cutting, then store at room temp. till I
need them. they go in a 60 degree oven for 30 minutes just prior to
deparaffinization in xylene. Any suggestions as to how to keep sections on
the slides when using severe antigen retrieval methods would be greatly
appreciated. Thanks.
Joyce Kotzuk
Univ. of New Mexico pathology

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