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From:Ian <ib@jeack.com.au>
Date:Fri, 12 Mar 1999 19:16:02 +1000
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Tenascin can be demonstrated in 10% NBF fixed tissue with a 1/100 to 1/200
dilution (Tris) of antibody (Dako MO636) and detected with a peroxidase
labeled streptavidin/ biotin system. I used the Dako LSAB+ with DAB as the
chromagen (REF: Nephrology 1995;1,291-295). I also included a 2 min
proteinase K prior to immunolabeling. Hope this helps. Ian Birchall.

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