Tenascin
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From: | Ian <ib@jeack.com.au> |
To: | HistoNet@Pathology.swmed.edu |
Reply-To: | |
Date: | Fri, 12 Mar 1999 19:16:02 +1000 |
Content-Type: | text/plain; charset="us-ascii" |
Tenascin can be demonstrated in 10% NBF fixed tissue with a 1/100 to 1/200
dilution (Tris) of antibody (Dako MO636) and detected with a peroxidase
labeled streptavidin/ biotin system. I used the Dako LSAB+ with DAB as the
chromagen (REF: Nephrology 1995;1,291-295). I also included a 2 min
proteinase K prior to immunolabeling. Hope this helps. Ian Birchall.
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